CLINICAL-THERAPEUTICAL MANAGEMENT OF EXPOSED PULP USING BIOACTIVE MATERIALS: TWO CASE REPORTS

Aims: This article present alternatives of bioactive materials use for direct pulp capping in immature teeth and for apexogenesis.Case report: Two case reports with the application of bioactive cementsare presented here: one in a tooth with pulp exposure and another with deep carious lesion, pulp exposure and open apex.Results: In both cases, the treatment was performed in one visit and a favorable clinical and radiographic follow-up was achieved.Conclusion: The use of bioactive materials ledto the formation of a dentin bridge and healing process in the pulp tissue exposure, without the need for conventional endodontic treatment

Light-curing of calcium hydroxide-based liners: pH analysis and calcium ion release / Fotoativação de forradores a base de hidróxido de cálcio: análise de pH e liberação de íons cálcio

Objective: Compare the pH values and calcium ion release of calcium hydroxide-based liner materials before and after light-curing. Material and Methods: The materials evaluated were: hydrox-cal white (HW), hydrox-cal dentin (HD), Biocal (BC) and UltraBlend Plus (UB). 120 samples of the liner materials were inserted into a PVC tube (n=15). The samples from HW+A, HD+A, BC+A and UB+A were subjected to photoactivation. The other groups HW+N, HD+N, BC+N and UB+N were only inserted in a glass tube with deionized water. The pH was measured 24 hours and 14 days after the inclusion of the samples with the aid of a pH meter. The calcium release was analyzed with the aid of an atomic absorption spectophotometer at 24h and 14 days. The results were submitted to the Shapiro-Wilk test, followed by ANOVA and Tukey test (p=0.05). Results: In 24h, the groups that were not light cured showed the highest pH values (p<0.05). In 14 days, BC+N and BC+A demonstrated the lowest pH values. The groups that were not light cured also showed higher calcium release values in 24h and 14 days (p<0.05). Conclusion: Photoactivation of calcium hydroxide-based liner materials negatively interferes with calcium ion release, as well as with pH.(AU)

Objetivo: Comparar os valores de pH e liberação de íons cálcio de materiais forradores à base de hidróxido de cálcio antes e depois da fotopolimerização. Material e métodos: Os materiais avaliados foram: Hidrox-cal branco (HW), Hidrox-cal dentina (HD), Biocal (BC) e UltraBlend Plus (UB). 120 amostras dos materiais de revestimento foram inseridas em um tubo de PVC (n=15). As amostras de HW +A, HD+A, BC+A e UB+A foram submetidas à fotoativação. Os demais grupos HW +N, HD+N, BC+N e UB+N foram inseridos apenas em um tubo de vidro com água deionizada. O pH foi medido 24 horas e 14 dias após a inclusão das amostras com o auxílio de um medidor de pH. A liberação de cálcio foi analisada com o auxílio de um espectrofotômetro de absorção atômica em 24h e 14 dias. Os resultados foram submetidos ao teste de Shapiro-Wilk, seguido de ANOVA e teste de Tukey (p=0,05). Resultados: Em 24h, os grupos não fotopolimerizados apresentaram os maiores valores de pH (p<0,05). Em 14 dias, BC+N e BC+A apresentaram os menores valores de pH. Os grupos não fotopolimerizados também apresentaram maiores valores de liberação de cálcio em 24h e 14 dias (p<0,05). Conclusão: A fotoativação de materiais de revestimento à base de hidróxido de cálcio interfere negativamente na liberação de íons cálcio e no pH (AU)

Addition of MTA in a salicylate-based resin root canal sealer to retrograde root-end filling

Aim To evaluate the flow, pH and calcium release of white MTA (WMTA), salicylate-based resin root canal sealer (Sealapex; SEAL) and SEAL containing 10 (SE10) or 20% (SE20) (w/w) of MTA. Methodology Flow test was realized according to ISO 6876 specification. The sealers samples (n= 10) were placed in polyethylene tubes and immersed in deionized water. After 24 hours and 7, 14, and 28 days, the water pH was determined with a pH meter, and calcium release was assessed by atomic absorption spectrophotometry. Results SEAL and WMTA showed respectively the highest and lowest flow rate when compared with the other materials (P<0.05). SE20 showed the highest pH value only in 24h and 7 days periods (p<0.05). In 14- and 28-days periods, SEAL showed the lowest pH value (p<0.05), but there are no differences between other groups (p>0.05). In all periods, WMTA and SEAL respectively showed the highest and lowest calcium release (p<0.05). Conclusions SE20 proves to be an association with better flow and handling than WMTA, with satisfactory potential for alkalinization and calcium release.

Antimicrobial activity of three temporary endodontic coronal sealers / Atividade antimicrobiana de três seladores coronários temporários em Endodontia

Objective: The object of the present study is to analyze, in vitro, the antimicrobial activity of three temporary endodontic coronal sealers.Methods: The materials tested were Tempo® (Vigodent, Rio de Janeiro, Brazil), IRM® (Dentsply, Petrópolis, Brazil) and Coltosol® (Vigodent, Rio de Janeiro, Brazil). The agar diffusion method was used for this analysis. Nine plates containing the agar blood culture medium were inoculated withhuman saliva and in each plate three equidistant cavities were made and filled with one of the materials tested. Two plates were not inoculatedand served as the negative control of the culture medium. All the plates were incubated in bacteriological incubators, in aerobiosis, for 48 hours, at 37oC. The inhibition halos of bacterial growth were measured in millimeters. Results: Tempo® (Vigodent, Rio de Janeiro, Brazil) did not show a inhibition halo of bacterial growth in any of the nine plates. Coltosol® (Vigodent, Rio de Janeiro, Brazil) produced halos in all plates, and IRM® (Dentsply, Petrópolis, Brazil) in 4 out of 9 plates. In all the tests, the halos produced by Coltosol® (Vigodent, Rio de Janeiro, Brazil) were more pronounced than the ones produced by IRM® (Dentsply, Petrópolis, Brazil) (p<0.05). The temporary coronal sealer Coltosol® (Vigodent, Rio de Janeiro, Brazil) presented the most prominent antimicrobial activity, followed by the temporary coronal sealer IRM® (Dentsply, Petrópolis, Brazil). Conclusion: Among the materials tested, it was concluded that the Coltosol® (Vigodent, Rio de Janeiro, Brazil) presented the highest antimicrobial activity.

Objetivo: Avaliar, in vitro, a atividade antimicrobiana de três seladores coronários temporários utilizados em Endodontia. Métodos: Os materiais testados foram o Tempo® (Vigodent, Rio de Janeiro, Brasil), IRM® (Dentsply, Petrópolis, Brasil) e Coltosol® (Vigodent, Rio de Janeiro, Brasil). O método utilizado para a avaliação foi o teste de difusão em ágar. Nove placas contendo o meio de cultura ágar-sangue foram inoculadas com saliva humana e em cada uma foram confeccionados 3 furos eqüidistantes sendo cada um preenchido com um dos materiais testados. Duas placas foram inoculadas e serviram como controle negativo do meio de cultura. Todas as placas foram incubadas em estufa bacteriológica, em aerobiose, por 48 horas, à temperatura de 37ºC. Os halos de inibição do crescimento bacteriano foram medidos em milímetros.Resultados: O selador temporário Tempo® (Vigodent, Rio de Janeiro, Brasil) não apresentou halo de inibição do crescimento bacteriano nas nove placas. Coltosol® (Vigodent, Rio de Janeiro, Brasil) produziu halo de inibição em todas as placas enquanto que o IRM® (Dentsply, Petrópolis, Brasil) produziu halo em 4 das 9 placas. Em todos os testes, os halos produzidos pelo Coltosol® (Vigodent, Rio de Janeiro, Brasil) foram maispronunciados que os do IRM® (Dentsply, Petrópolis, Brasil) (p<0.05). O selador coronário temporário Coltosol® (Vigodent, Rio de Janeiro,Brasil) apresentou a atividade antimicrobiana mais pronunciada, seguido pelo IRM® (Dentsply, Petrópolis, Brasil). O selador temporário Tempo®(Vigodent, Rio de Janeiro, Brasil) não apresentou qualquer atividade antimicrobiana. Conclusão: Dentre os materiais testados, podemos concluir que o Coltosol® (Vigodent, Rio de Janeiro, Brasil) é o que apresenta maior atividade antimicrobiana.